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Attia, K.
- Establishment of Mature Axillary Bud Culture of Sugarcane and overcoming Persistent Culture Contamination
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PDF Views:246
Authors
Affiliations
1 Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB, CA
2 Department of Agriculture, The University of Queensland, Brisbane, Qld 4072
3 Institute of Genetic Engineering, Fudan University, Shanghai, 200433, CN
1 Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB, CA
2 Department of Agriculture, The University of Queensland, Brisbane, Qld 4072
3 Institute of Genetic Engineering, Fudan University, Shanghai, 200433, CN
Source
Indian Journal of Science and Technology, Vol 2, No 1 (2009), Pagination: 18-25Abstract
Several methods to eradicate contaminants from sugarcane mature axillary budculture including bud location/age, surface sterilization and thermal and chemical treatments of buds are examined. A spectacular improvement in the decontamination rate to 95.8 % was obtained when the heat treatment 61 °C for 30 min. was initially applied, followed by either 52 or 57 °C for 30 min, 24 hours apart. The various methods investigated and the successful rates of decontamination of sugarcane axillary buds may have a wider application in other plant tissue culture systems with known persistent contamination.Keywords
Axillary Bud Culture, Tissue Culture Contamination, Disease Free Stock, SugarcaneFull Text
References
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- Improved Production of the Indole Alkaloid Canthin-6-one from Cell Suspension Culture of Brucea javanica (L.) Merr.
Abstract Views :357 |
PDF Views:142
Authors
Affiliations
1 Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB E2L 4L5, CA
2 Department of Pharmacy, Faculty of Natural Sciences, Hasanuddin University, ID
3 Institute of Genetic Engineering, Fudan University, Shanghai, 200433, CN
1 Department of Biology, Faculty of Science, University of New Brunswick, Saint John, NB E2L 4L5, CA
2 Department of Pharmacy, Faculty of Natural Sciences, Hasanuddin University, ID
3 Institute of Genetic Engineering, Fudan University, Shanghai, 200433, CN
Source
Indian Journal of Science and Technology, Vol 1, No 7 (2008), Pagination: 1-6Abstract
In an attempt to increase the productivity of the pharmaceutical compound canthin-6-one from cell suspension culture of Brucea javanica, a high cell density culture and improved culture conditions have been investigated with focus on culture conditions and stages of growth. The highest yield of canthin-6-one, 26.72 mg g-l dry cell, was produced intracellular in 50 g L-1 cell mass. The established cell suspensions were harvested at the age of 40 days and placed into MS medium containing 1.0 mg L-1> 2,4-D, 1.0 mg L-1 NAA, 0.1 mg L-1 Kinetin and 10 mg L-1 tryptophan for 33-40 days, approximately when the cultures were at their late stationary phase of growth. Addition of 20 mg L-1 tryptophan did not show a significant difference in canthin-6-one production (p>0.05). Higher than 10 mg L-1 of tryptophan showed adverse effect on the concentration of canthin-6- one alkaloid (p=0.01). Qualitative analysis of chloroform-extracts of dried cells from suspension cultures revealed spots on TLC; and the identity of canthin-6-one was established by comparison of its Rf value (Rf=0.37), its colour reaction with Dragendorff reagent and ammonium sulphate and its behaviour in UV with an authentic sample. Quantification of canthin-6-one alkaloid using TLCdensitometry scanner in response to different concentration of tryptophan as a precursor showed a concentration-dependent response. The production of canthin-6-one at 26.72 mg g-1 dried cell weight in this study, establishes a methodology of an improved procedure compared to those previously reported and offers an opportunity for production of canthin-6-one alkaloid at an industrial level.Keywords
Canthin-6-one, Cell Suspension, Brucea Javanica, Secondary MetabolitesFull Text
References
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